mouse intesticult organoid growth medium  (STEMCELL Technologies Inc)

Journal: Animals : an Open Access Journal from MDPI

Article Title: IL-1β Induced Intestinal Inflammation Pathogenesis in East Friesian Sheep: Insights from Organoid Modeling

doi: 10.3390/ani15081097

Figure Lengend Snippet: Sheep enteroids cultured in an Intesticult medium alone. ( a ) Representative images showing the growth and development of individual enteroids over 9 days of culture. Scale bar, 100 μm. ( b ) Second-generation growth state after passaging (P1: passage 1; D1: day 1 post-passage). Scale bar, 100 μm. ( c ) Growth status of organoids across days in the first three generations. ( d ) Statistical analysis of 9-day area changes in first-generation organoids (ANOVA, p < 0.05). ( e ) Statistical analysis of 7-day germination rates for first-generation organoids (ANOVA, p < 0.01). ( f ) Germination rate comparison among the first three generations (ANOVA, p < 0.05). Scale bar, 100 μm. (Concentrate: d–f: Individual data plots are presented. Data are derived from three individual organoids distributed among three individual wells. These wells are from one different culture batch. A total of one animal was used in the experiment to generate the raw counts shown in the graphs.).

Article Snippet: Plates were incubated at 37 °C, 5% CO 2 for 30 min to allow the Matrigel to polymerize and then 500 μL of prewarmed complete IntestiCult Growth Medium (mouse) (6005; STEMCELL Technologies, Vancouver, BC, Canada) containing 10 μM Y-27632 (HY-10071; MCE, Shanghai, China), 10 μM LY2157299 (SF7926; Beyotime, Shanghai, China) [ ], 25 μg/mL FGF-basic (Z03230; GenScript, Nanjing, China), 100 ng/mL IGF-1 (Z03688; GenScript, Nanjing, China), Gen-2.7%, and P/S-1% added to each well.

Techniques: Cell Culture, Passaging, Comparison, Derivative Assay

Journal: iScience

Article Title: A bacterial genotoxin reveals a p53-proteasome-LC3 regulatory axis that drives the suppression of autophagy in cells experiencing sublethal DNA damage

doi: 10.1016/j.isci.2025.112118

Figure Lengend Snippet:

Article Snippet: IntestiCult Mouse Organoid Growth Medium , Stem Cell Technologies, Canada , Cat#06005.

Techniques: Virus, Recombinant, Cell Recovery, Modification, Gentle, Flow Cytometry, Reverse Transcription, Bicinchoninic Acid Protein Assay, RNA Extraction, Western Blot, Cloning, Mutagenesis, Plasmid Preparation, Software, Cell Culture, Microscopy, Nucleic Acid Electrophoresis